Part-Time Lab Technician

St. Johns established in 1870 hastwoNew York City campuses; international locations in Rome Italy; Limerick Ireland; and Paris France; and study abroad locations around the world. The Princeton Review and other top rankings consistently recognize the Universitys outstanding academics diverse student body dynamic internship and volunteer opportunities focus on student life and diverse study abroad offerings. St. Johns University offers more than 100 undergraduate and graduate programs in its six colleges and schools with a growing number of programs offered online. The University is accredited by the Middle States Commission on Higher Education and 12 other major academic and professional associations.

A dedication to diversity equity and inclusion is at the heart of our mission. As a Catholic and Vincentian university St. Johns is committed to institutionalizing practices of inclusive excellence to ensure that we welcome and celebrate the intrinsic worth of all members of our community. We will become an even stronger university as we enhance equity at every level of our graduates will excel in the competencies and values needed for leadership and service in a rapidly evolving world.

Department : Pharmaceutical Sciences

Specific Job Title : Part -T ime L ab T echnician (1 position is available)

Reports to : Sei Higuchi Ph.D. ()

Campus : Queens

Job Summary:

Responsibilities are 1) assisting with experiment s 2) managing lab inventory and 3) making experiment material . This lab technician position is expected to assist with experiments for Ph .D. Students Undergraduate students and PI. Applicants need basic knowledge of molecular biology biochemistry and pharmacology.

Essential Functions:

Major responsibilities:

Assisting with experiments : Assisting with experimental procedures including gel electrophoresis quantitative PCR (qPCR) cell culture and mouse genotyping.

Managing In ventory: Ma intaining laboratory supplies reagents and equipment to ensure an efficient research environment.

Making e xperiment material : Preparing culture media Phosphate-buffered saline (PBS) buffers for experiment (western blotting running buffer western blotting transfer buffer animal perfusion buffer) 0.9% NaCl saline and sterile experiment materials (water for incubator surgical materials for animals 1.5 tubes for cell culture). Decontamination of research equipment.

Reporting : The Lab technician need s to report the activity to the PI weekly .

Competencies:

Required skills: DNA extraction agarose gel making electrophoresis DNA band imaging PCR primer preparation buffer (PBS western blotting running buffer and western blotting transfer buffer etc ) making sterile procedures cell culture media making and lab material inventory check .

Work Environment:

The laboratory technician will work in room 61 St. Albert Hall St. Johns University Queens Campus. Dr. Sei Higuchis laboratory (approximately 840 square feet in size) is part of the College of Pharmacy and Health Sciences of St. Johns University and is located on the main campus in Jamaica NY. The laboratory is located on the B-level of St. Albert Hall (SAH.B61). The laboratory is equipped with a functional tissue culture hood for work with microorganisms and a 37-degree Celsius CO2-regulated tissue culture incubator. This lab space is equipped with a Class II biosafety cabinet. PIs laboratory has six working benches two benches for equipment and a desk for five-six students and lab technician along with a computer and a printer. It also is equipped with a Class II biosafety cabinet and a chemical fume hood ducted to the roof of St. Albert Hall. The laboratory has the following additional equipment; qPCR System QS3 Nanodrop one thermal cycler ( Thermo Fisher) plate reader SpectraMax M2 Centrifuge (Eppendorf 5424R 5804R and VWR1814) four Voltex machines auto cell counter Countess II FL (Life Technologies) -80 oC freezer (13 Cu) two -20 oC freezer x2 (4.3Cu) four fridges (4.3Cu) Milli-Q water purification system water bath two analytical balances three inverted microscopes pH meter Seahorse Extracellular Flux Analyzer tissue homogenizer (bead mill and generator probe homogenizer) power supplies and two sets of western blotting equipment (Invitrogen).

Physical Demands:

The l ab technician need s to make a maximum of 1-5 L buffer and handle research equipment including PC .

Environmental Conditions:

The lab has some noise from the freezer and the timer for the experiment. Cell culture incubators contain 5% CO2. The lab has some biohazardous chemical compounds.

Position Type/Expected Hours of Work:

The lab technician will be expected to work 20 hours per week. Working days and times are flexible.

Required Education and Experience:

A bachelors degree is .

Preferred Education and Experience:

Preferably a Pharm D or equivalent degree.

In compliance with NYCs Pay Transparency Act the hourly salary range for this position is $22. 00 - $25.00 .

*ImportantNote- St. Johns is NOT anE-VerifyEmployer

St. Johns offers a competitive compensation program which is commensurate with your qualifications experience and contingent upon the departmental budget. We also offer an extremely comprehensive benefits program to meet the diverse needs of our workforce. Along with exceptional benefits such as medical dental life insurance long term disability insurance tuition remission generous 403(b) employer contribution employee assistance program and liberal paid time off policies faculty and staff can also enjoy St. Johns performing arts libraries bookstores dining facilities campus recreation and sporting events

Any offer of employment is subject to receipt by St. Johns University of satisfactory references verification of employment and education.

St. Johns University is an Equal Opportunity qualified applicants will receive consideration without regard to race color religion sex sexual orientation gender identity national or ethnic origin age status as an individual with a disability protected veteran status or any other characteristic protected by law.

Required Experience:

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